Research Project: Structure determination of Calmodulin in complex with C21W and C20W using 3-dimensional NMR experiments.
Calmodulin is a dumbbell shaped protein with four Calcium binding sites. It plays an important
role as a calcium regulatory protein. In the presence of calcium it binds among other to
plasma membrane ATPase. The peptides C20W and C21W resemble different parts of the CaM binding site of the
plasma membrane ATPase. Small angle X-ray measurements suggest that C20W binds only to one
domain of CaM, whereas C21W binds to both domains, causing the dumbell shape of calmodulin to collapse
to form a more globular structure. The goal of the current project is to compare the structures
of the two complexes in solution using NMR techniques.
Structure of CaM/C20W
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